Unlimited real-time PCR analysis from precious RNA samples
May 30 2007

Unlimited real-time PCR analysis from precious RNA samples

May 30, 2007 The QuantiTect Whole Transcriptome Kit delivers high and reproducible cDNA yields from limited amounts of RNA by whole transcriptome amplification (WTA). The cDNA is intended for use in real-time PCR and contains uniformly amplified targets to ensure reliable gene expression analysis.

Comprehensive gene expression analysis of rare samples is often not possible due to low RNA amounts. The QuantiTect Whole Transcriptome Kit overcomes this by amplifying all mRNA transcripts in a sample. From as little as 1 ng RNA or RNA corresponding to about 50 cells, up to 40 µg cDNA is generated for unlimited real-time PCR analysis and archiving.

Highly uniform amplification of all mRNA transcripts is achieved with the QuantiTect Whole Transcriptome Kit. Both the 5' and 3' regions of each transcript are equally amplified, and the relative abundance of each transcript is preserved after amplification. This ensures unbiased results in subsequent gene expression analysis.

Whole transcriptome amplification is performed in a streamlined 3-step protocol. RNA is first reverse transcribed into cDNA and then ligated before being amplified using proven REPLI-g technology. The QuantiTect Whole Transcriptome Kit contains all the necessary enzymes and buffers for the process, and does not require reaction optimization by the user.

The QuantiTect Whole Transcriptome Kit is intended for research use. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

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