Ni-NTA Superflow now available in FPLC-compatible cartridges
Ni-NTA Superflow from QIAGEN - the resin of choice for purification of His-tagged proteins - is now available in pre-filled 1 ml and 5 ml cartridges for purification on liquid chromatography platforms, such as the FPLCTM, ÄKTATM, and BioLogicTM systems.
Ni-NTA matrices are the most-cited solution for purifying 6x His-tagged proteins. Their high stability means that they are compatible with a wide range of buffer components, including strong denaturants, detergents, and even reducing agents. This flexibility enables researchers to develop optimal purification schemes while still benefiting from the excellent separation characteristics delivered by Ni-NTA, and often makes a second chromatographic step unnecessary. An independently performed comparison with other commercially available nickel resins demonstrates that the tetradentate NTA ligand used in Ni-NTA shows the lowest level of nickel leaching - minimizing nickel contamination of protein preparations and preventing the remaining charged ligands from binding non-tagged proteins that will contaminate elution fractions.
Ni-NTA Cartridges are used to purify from 5 -100 mg His-tagged protein. The robust Superflow matrix allows high flow rates, speeding the purification procedure and increasing throughput. The cartridges are quickly and easily connected to liquid chromatography systems and the purification process is highly reproducible, guaranteeing the same high quality protein preparations time after time.
Trademarks: QIAGEN® (QIAGEN Group); ÄKTATM, FPLCTM (Amersham Biosciences); BioLogicTM (Bio-Rad Laboratories).