QuantiFast Kits — fast PCR on any real-time cycler
Jan 23 2007

QuantiFast Kits — fast PCR on any real-time cycler

January 23, 2007

QuantiFast Kits deliver significantly faster results in real-time PCR. With time savings of up to 60% on existing cyclers, there is no need for costly hardware upgrades. In addition, no PCR optimization is required, even if using previously established assays. Kits are available for SYBR Green or probe-based detection in PCR, two-step RT-PCR, and one-step RT-PCR.

A specially developed fast PCR buffer containing the patent-pending additive Q-Bond greatly reduces denaturation, annealing, and extension times. The buffer also contains HotStarTaq Plus DNA Polymerase, which requires only 3 or 5 minutes at 95ºC for activation. For one-step RT-PCR, the optimized QuantiFast RT Mix enables cDNA synthesis in just 10 minutes.

Accurate quantification of even low copy numbers are achieved with QuantiFast Kits. A balanced combination of KCl and NH4Cl in the fast PCR buffer promotes specific primer annealing, enabling high PCR specificity and sensitivity. In addition, HotStarTaq Plus DNA Polymerase provides a stringent hot start, preventing the formation of nonspecific products.

QuantiFast Kits are ready-to-use master mixes that eliminate the need for optimization of reaction and cycling conditions. Simply follow the handbook protocol to get fast and reliable results. The kits are compatible with cyclers that use ROX dye for fluorescence normalization (no optimization of ROX concentration is necessary) and with cyclers that do not.

Novel Q-Bond technology is also used by the QIAGEN Fast Cycling PCR Kit, which is designed for standard and challenging end-point PCR applications. The kit provides fast and specific PCR on standard as well as fast-ramping thermal cyclers. Significant time savings of up to 75% can be achieved.

QuantiFast Kits and the QIAGEN Fast Cycling PCR Kit are intended for research use. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.


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