QIAGEN Introduces Innovative Kits for Large-Scale Plasmid DNA Isolation
February 26, 2008 Two new products for large-scale plasmid DNA isolation are being introduced by QIAGEN, the leading supplier of sample and assay technologies. The CompactPrep Plasmid Mega and Giga kits offer greater convenience and faster extraction than other methods. The purification capacity is up to 2.5 mg of plasmid DNA for the Mega Kit and up to 10 mg for the Giga Kit.
With commonly used techniques, isolation of large quantities of plasmid DNA was often very time consuming, requiring as much as several hours to complete. This posed significant challenges for researchers facing increasing time pressures. In contrast, the new kits allow isolation of plasmid DNA in less than 50 minutes due to a vacuum-driven protocol. In addition, by using the company's QIAvac 24 Plus vacuum device in combination with the new QIAvac Holder, it is possible to process up to 12 preparations in parallel. Virtually no hands-on work is required during the entire procedure.
Another advantage of the CompactPrep Plasmid Mega and Giga kits is the highly concentrated, low-endotoxin plasmid DNA produced. The new kits use small columns that enable elution of DNA in a very low buffer volume, resulting in DNA concentrations of up to 2.5 μg/μl. In addition, by using a supplemental wash buffer, endotoxin levels are usually lower than 1 EU/μg DNA.
The resulting purified plasmid DNA is suitable for all routine downstream applications, including cloning, enzymatic restriction, in vitro transcription and translation. Due to the kits' proprietary wash buffer, the extracted plasmid DNA is ideal for transfection experiments, even with cell lines as sensitive as Huh-7.
"With the CompactPrep Plasmid Mega and Giga kits, our customers will benefit from a reduction in preparation time of up to 80 percent for large-scale purification, compared to present methods," said Dr. Nina Gildehaus, Associate Global Product Manager of Plasmid DNA Purification at QIAGEN. "This means they will be able to significantly improve their productivity - a great advantage in times of increasing time pressure."
About CompactPrep Plasmid Mega and Giga kits:
- Are fastest available for large-scale plasmid preparation: 40 minutes for the Mega Kit (up to 2.5 mg plasmid DNA), 50 minutes for Giga Kit (up to 10 mg plasmid DNA).
- Offer high-throughput option: can process up to 12 preparations in parallel on the QIAvac 24 Plus vacuum manifold device.
- Produce highly concentrated, ready-to-use plasmid DNA (up to 2.5 μg/μl).
- Feature high-purity output with endotoxin levels of usually less than 1 EU/μg DNA.
Pictures are available at www.qiagen.com/goto/compactprepimages/
QIAGEN N.V., a Netherlands holding company, is the leading provider of innovative sample and assay technologies and products. QIAGEN's products are considered standards in areas such as pre-analytical sample preparation and assay solutions for life sciences, applied testing and molecular diagnostics. QIAGEN has developed a comprehensive portfolio of more than 500 proprietary, consumable products and automated solutions. The company's products are sold to academic research markets, leading pharmaceutical and biotechnology companies, applied testing customers (such as in forensics, veterinary, biodefense and industrial applications) and molecular diagnostics laboratories. QIAGEN products are sold through a dedicated sales force and a global network of distributors in more than 40 countries; the company employs more than 2,600 people worldwide. Further information about QIAGEN can be found at http://www.qiagen.com/.